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Substrate solution is then added and color develops in proportion to the amount of Rat HYAL1
it binds with CXCL16 bound to precoated antibody
only those wells that contain Human PIIINP
IRF8 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse gABRa2
Human PSPN (Persephin) ELISA Kit IL1-R1 Substrate solution is then addedThe Human PSPN (Persephin) ELISA Kit measures PSPN in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PSPN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
